Flow cytometry intracellular staining

Hello everyone, I am new here and I am looking for help for my flow cytometry experiments. It would be very nice if someone could help me out.

I am working on a project where I want to stain intracellular. For this I need to fix and permeabilize cells before doing the flow cytometry experiment. To achieve this I am currently using a cocktail containing 4% PFA and 0.2% triton X-100 in PBS and I incubate cells for 10 min at room temperature. After doing the staining and multiple washing steps I will not get a nice populations in my flow cytometry data, I see a lot of debris and the cell count is very low…

Does someone have any tips? All help is welcome!